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assembly software package  (Bioedit Company)

 
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    Structured Review

    Bioedit Company assembly software package
    Assembly Software Package, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/assembly+software+package/assembly+software+package/pm30339222-60-7-6
    Average 90 stars, based on 1 article reviews
    assembly software package - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Reductive dechlorination of high concentrations of chloroethenes by a Dehalococcoides mccartyi strain 11G.
    Article Snippet: 16S rRNA gene inserts representing distinct enzyme restriction patterns were sequenced at Integrated DNA Technology, Singapore. .. Sequencing results were assembled using the BioEdit assembly software package (http://www.mbio.ncsu.edu/BioEdit/bioedit.html) and analyzed with BLASTn (http://www.ncbi.nlm.nih.gov/). ..

    Software:

    Article Title: Reductive dechlorination of high concentrations of chloroethenes by a Dehalococcoides mccartyi strain 11G.
    Article Snippet: 16S rRNA gene inserts representing distinct enzyme restriction patterns were sequenced at Integrated DNA Technology, Singapore. .. Sequencing results were assembled using the BioEdit assembly software package (http://www.mbio.ncsu.edu/BioEdit/bioedit.html) and analyzed with BLASTn (http://www.ncbi.nlm.nih.gov/). ..

    Article Title: Microbial reductive dehalogenation of trihalomethanes by a Dehalobacter-containing co-culture.
    Article Snippet: Trihalomethanes such as chloroform and bromoform, although well-known as a prominent class of disinfection by-products, are ubiquitously distributed in the environment due to widespread industrial usage in the past decades.. Chloroform and bromoform are particularly concerning, of high concentrations detected and with long half-lives up to several hundred days in soils and groundwater.. In this study, we report a Dehalobacterand Desulfovibriocontaining co-culture that exhibits dehalogenation of chloroform (~0.61 mM) to dichloromethane and bromoform (~0.67 mM) to dibromomethane within 10–15 days.



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    HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp <t>(HBV</t> <t>sequence</t> 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. Ligated DNAs introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using <t>SeqMan</t> assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.
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    Image Search Results


    HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. Ligated DNAs introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using SeqMan assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.

    Journal: Antiviral research

    Article Title: Distinct dual antiviral mechanism that enhances hepatitis B virus mutagenesis and reduces viral DNA synthesis

    doi: 10.1016/j.antiviral.2019.104540

    Figure Lengend Snippet: HepAD38 cells (1.0 X 105 cells per well) were plated in a 24-well plate 24 h prior to virus-producing induction. Five days following removal of tetracycline from cultured HepAD38 cells and cell treatment with 10 nM gemcitabine, 50 μM 5-aza-dC, 200 μM 5-aza-C or the combination of 5-aza-dC/5-aza-C with gemcitabine, HBV rcDNA was purified from cell culture supernatants. A DNA product of 714 bp (HBV sequence 87–800) was amplified by PCR. After gel purification, the 714 bp product was ligated to the pGEM-T vector. Ligated DNAs introduced into E. coli by transformation, and insert containing-vectors were subjected to DNA sequencing, with 70 −100 colonies were sequenced per each treatment (see Supplemental Table 1). Sequence alignments were done by using SeqMan assembly tool in the Lasergene 7 software package (DNAStar; Madison, WI). A. Mutation frequency analysis. Mutation frequency was calculated by the number of mutations divided by the total number of bases sequenced. The mutation frequency of the untreated was 1.46 × 10−4 mutations per basepair (see Supplemental Table 1). B. Mutation spectra analysis. The spectra of mutations from each experimental group is depected by a pie chart, which shows the main mutation types and percentages observed. The size of the pie chart was normalized to the mutation frequency of untreated controls. Results are shown from 3 independent experiments. The gray asterisks and brackets associating any two bars indicate the significant difference between the experimental pairs. The statistical significance compared to the untreated control is shown above the bar with black asterisks. Significance was analyzed with the “N-1” Chi-squared test; “ns” = not significant; “****” = p ≤ 0.0001; “**” = p ≤ 0.01; “*” = p ≤ 0.05. Abbreviation: gem = gemcitabine.

    Article Snippet: Sequence alignments were performed using SeqMan assembler of Lasergene 7 software package (DNAStar; Madison, WI).

    Techniques: Cell Culture, Purification, Sequencing, Amplification, Gel Purification, Plasmid Preparation, Transformation Assay, DNA Sequencing, Software, Mutagenesis